Intrapartum PCR-assay for detection of Group B Streptococci (GBS)
نویسندگان
چکیده
منابع مشابه
Detection of group B streptococci (GBS) in vaginal swabs using real-time PCR with TaqMan probe hybridization.
BACKGROUND & OBJECTIVES Implementation of a screening based strategy for the prevention of perinatal group B streptococcus (GBS) disease is anticipated to increase the demand of fast laboratory techniques. The aim of the present study was to develop a real-time PCR method for the specific detection of GBS in vaginal swabs. METHODS Based on partial sequencing of the sip gene, primers and a Taq...
متن کاملReal-time PCR assay provides reliable assessment of intrapartum carriage of group B Streptococcus.
The objective of this study was to determine the reliability of the real-time PCR assay for determining the group B Streptococcus (GBS) status of women in labor. In this prospective study we compared the results of culture and PCR testing of vaginal and rectal samples collected by nursing staff when women were in labor. Patients' charts were also reviewed to obtain relevant information about pr...
متن کاملDevelopment of conventional and real-time PCR assays for the rapid detection of group B streptococci.
BACKGROUND Group B streptococci (GBS), or Streptococcus agalactiae, are the leading bacterial cause of meningitis and bacterial sepsis in newborns. Currently available rapid methods to detect GBS from clinical specimens are unsuitable for replacement of culture methods, mainly because of their lack of sensitivity. METHODS We have developed a PCR-based assay for the rapid detection of GBS. The...
متن کاملIntrapartum PCR assay versus antepartum culture for assessment of vaginal carriage of group B streptococci in a Danish cohort at birth
The aim of this study was to compare the performances of two strategies for predicting intrapartum vaginal carriage of group B streptococci (GBS). One strategy was based on an antepartum culture and the other on an intrapartum polymerase chain reaction (PCR). We conducted a prospective observational study enrolling 902 pregnant women offered GBS screening before delivery by two strategies. The ...
متن کاملComparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor
Objective(s) Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and Methods Two swabs were used to obta...
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ژورنال
عنوان ژورنال: European Journal of Obstetrics & Gynecology and Reproductive Biology: X
سال: 2019
ISSN: 2590-1613
DOI: 10.1016/j.eurox.2019.100081